Fluorescence analysis is a highly sensitive and high selective sophisticated analytical method. This method can provide information including excitation and emission spectrum, emission light intensity and measurement of life of emission light and polarization fluorescence, etc. This method can provide a wide linear range of the working curve. It has become an important analytical method in the region of trace analysis. This method has been used in:

• Medical science and clinical analysis of the biological specimens.
• Pharmaceutical science and pharmacology Analysis of natural pharmaceutical products; Quality control of pharmaceuticals and research of pharmaceutical metabolites.
• Biochemistry Analysis of minute quantity of substances in a biological body.
• Food industry Analysis of minute quantity of constituents in food.
• Pollution analysis Atmospheric pollution, environmental testing, and food contamination analysis.
• Organic and inorganic chemistry are Used in the trace analysis in case of those substances cannot be determined by absorption spectrophotometry